Re: No New Messages & And a plant Chimera link
- Subject: Re: No New Messages & And a plant Chimera link
- From: andrewl <email@example.com>
- Date: Sun, 22 Apr 2001 08:10:23 -0500
Jim Hawes wrote:
No New Messages....
It's true, Jim. We avid Hostaholics know when it's time to get
off the computer and out into the garden! :-)
I did stumble onto a web site that has quite a bit of info about Sports
and Chimeras, particularly in the Tissue Culture process. Good reading
even if I do say so myself...
PLANT CHIMERAS IN TISSUE CULTURE: A REVIEW
Mary F. Pogany and R. Daniel Lineberger
I imagine this link has been posted before but it is a very nice review
even though not too specific about Hosta.
There is good info here about L1, LII, LIII nomenclature; the appearance
of Chimeras in the TC lab and from whence they originate' explanations
regarding results one can anticipate when use axillary shoots vs. adventitious
shoots (which I'm still working on understanding), etc. Not
much here on linkage mapping and transposable elements but it explains
things pretty well from a micro-biological point of view.
Incidentally, if anyone has this article, "Marcotrigiano, M. and R.N.
Stewart. 1984. All variegated plants are not chimeras. Science 223: 505",
I'd be most obliged for a chance to read it. Might even consider
a Hosta trade or two!
I've been spending some serious time with about 250 Hosta varieties
and am learning my lessons regarding culture, species and cultivars well.
One thing for sure, a commercial grower probably should NOT accept TC babies
real late in the season, unless equipped with HPS or MH lights and prepared
to keep the heat on for a long time into the winter. Had a lot of
casualties from the very last batch that I received in late 2000 (around
October 15?) It is amazing, however, how some of the teeniest
little meristems made it through the winter and have bounced back.
And the more established plants have come up with a conviction to grow,
grOW, GROW, which we of course like. This is not to say that young
TC babies would not survive well in the out-of-doors, because my experience
has been that they survive quite well (if the squirrels don't dig them
out, the voles chew them up, or the rabbits nip them off to the roots early
in the spring). These later factors are DIFFERENT than what one faces
in a GH. Young plants in the garden may have to be protected untill
they are better established.
The highest causes for success? Use the proper potting mix and
keep them dry (Tom Michelleti was, is, and most likely will continue to
be, an honest man. Tom extolls this position frequently, and for
good reason), and then fix any potting mix problems BEFORE starting to
water in the spring. Sounds simple, doesn't it? Yepper, it
is, if implemented properly from the start.
It is interesting, however, how many did WELL in one casual experiment
I did. I placed about 15 dozen, that had been potted up
and grown on all season, out in open flats right where mother nature could
do whatever she chose to them. I did this with some not so expensive
varieties just to see how tough they were. I'll record some results
on this but the casualties were much more strongly correlated to variety
than to treatment. Some of you who have been growing Hostas for years
already knew that, but for we novices, it's an interesting revelation.
This procedure is RISKY and not recommended, but I was just being curious.
My current estimate of total necrosis is about 2-3% from the wintering
over endeavor. I'm keeping track of the hits, by cultivar, with
some diffentiating via conjecture as to cause of death and I'll have a
final tally in about one week. Like I said, a very high percentage
had to do with a carry over from using the wrong potting mix early last
spring (I had just not gotten around to repotting all of them).
Finally, "Don't take too much advice from an annuals grower if you're
growing perennials". Once again, "no duh", and at my age you'd think
I'd know better...!
Hosta la Vista!
#1 Plantsman at http://hostahaven.com
1250 41st St
Des Moines, IA 50311-2516