Date: Mon, 18 Jan 1999
Subject: Re: bulbs and colchicine
reprinted from: Alpine-L Digest
With all the talk of tetraploidy
inducements, and the great dangers involved with colchicine, let me pass along a
much safer method. Instead of using colchicine, researchers have found
that Surflan pre-emergent herbicide has the same effect as colchicine.
Researchers tell me that the mutation rate on plants is higher and much lower in
Dip 5-7 day old seedlings in a
solution of Surflan (High Rate is 1:100, while a low rate is 1:200). This
is using the standard store shelf solution to start. The seedlings should
remain in solution for 24 hrs. After this, they should be thoroughly rinsed for
3-4 hours under running water. Then plant and wait. While 50% should
die, most of the rest will be tetraploid.
We have had excellent success with
this method. Among the tets so far include ligularia, rohdea, and hosta. I
hope this is of use.
Raleigh, NC USA
Tue, 19 Jan 1999 reprinted from: Alpine-L Digest:
Tetraploids are reportedly induced
by the entire group of pre-emergent herbicides that include both Treflan and
Surflan. I'll have to rely on some more scientific types to explain how
this group works. A article titled "Drugs with Colchicine-like Effects
that Specifically Disassemble Plant but Not Animal Microtubules" outlines the
scientific stuff. This was published in The Annals of the NY Academy of
Sciences, 466:767, 784 (1986) by A.S. Bajer and J. Mole-Bajer Univ. of Oregon.
To date, there are three hostas on
the market now that are Treflan induce tetraploids, H. 'Patriot', H. 'Night
Before Christmas', and H. 'Grand Tiara'. It is my understanding that
the ploidy levels have been confirmed by Ben Zonneveld of Leiden, Netherlands.
High Rate of 1:100 using standard store
shelf solution (40%):
40% active ingredients is 40 grams surflan/100 ml
solution or 0.4 grams per 1 ml solution
1:100 would be 1ml solution + 99 ml
0.4 grams Surflan/100ml, or 0.4% Surflan Solution