hort.net Seasonal photo, (c) 2006 Christopher P. Lindsey, All Rights Reserved: do not copy
articles | gallery of plants | blog | tech blog | plant profiles | patents | mailing lists | top stories | links | shorturl service | tom clothier's archive0
 Navigation
Articles
Gallery of Plants
Blog
Tech Blog
Plant Profiles
Patents
Mailing Lists
    FAQ
    Netiquette
    Search ALL lists
    Search help
    Subscription info
Top Stories
Links
sHORTurl service
Tom Clothier's Archive
 Top Stories
Disease could hit Britain's trees hard

Ten of the best snowdrop cultivars

Plant protein database helps identify plant gene functions

Dendroclimatologists record history through trees

Potato beetle could be thwarted through gene manipulation

Hawaii expands coffee farm quarantine

Study explains flower petal loss

Unauthorized use of a plant doesn't invalidate it's patent

RSS story archive

Hardening Off Seedlings etc.

  • Subject: Hardening Off Seedlings etc.
  • From: "Dan & Lu" <hostanut@Bellsouth.net>
  • Date: Fri, 25 Jan 2002 22:20:05 -0500

Several years ago I had the pleasure of touring Pat Scolnik's tissue culture lab, Breeze Hill Farm.
 
It's much more difficult to harden off small tissue culture explants than it is seedlings.
 
Pat had a real interesting technique. She had plastic domes that covered her flats that allowed her to go from full dome cover to domes that had a few holes in them to domes that had more holes in them to domes that had lots of holes in them and finally to no dome at all. 
 
It was a real neat system and Pat was using it on hundreds of flats of hosta tissue culture liners. Pat was using this system to root stage four plantlets(just leaf....and no roots) and was rooting stage four hosta into promix like soil Vs. the regular agar medium used in tc labs for stage four rooting. She said her losses were a little higher but it eliminated a whole stage in the hosta tc process.
 
If you use the regular size greenhouse flats the plastic domes are cheap and it's easy to afford many domes for different ventilation levels.
 
Dan & Lu




 © 1995-2015 Mallorn Computing, Inc.All Rights Reserved.
Our Privacy Statement
Other Mailing lists | Author Index | Date Index | Subject Index | Thread Index