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Re: DNA and pollen viability in hosta

  • Subject: Re: DNA and pollen viability in hosta
  • From: halinar@open.org
  • Date: Sat, 2 Mar 2002 19:38:14 -0800 (PST)


>Therefore most standard deviations from the species are around 0.2% 
>as published. 

How do you get a standard deviation based as a percentage?  A standard 
devistion is just a measure of the variation of the mean around the 
mean and is expressed in the same units as are used in the data.

If you have a 0.2% error and hosta generally average about 30 pg DNA, 
that gives a standard deviation of 0.6 pg and a 95% confidence level 
of +/- 1.2 pg.  I believe you said most hosta are between 28 and 32 pg 
DNA.  A hosta measured at 28 pg could be 29.2 pg while a hosta with 30 
pg could be 28.8.  Your error rate is such that you need at least a 
2.4 pg difference bvetween any two hostas before you can consider them 
to be significently different.  Given that most hosta vary only 
between 28 and 32 pg, I don't see how the DNA data can be of any great 
value by itself.  At best all you can do is seperate the hosta species 
into maybe three DNA groups: low, medium and high DNA levels.

This isn't to say the DNA content isn't valuable information, but it 
is only valuable when combined with other information.

Joe Halinar
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