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Re: surflan and DNA content


>I use it as a simple way to find out or the hybrids of the tetraploid 
>ventricosa with any other diploid  plant are really triploid as they 
>should be.

I don't think there is any higher plant genera where you can't find 
unreduced gametes IF you look for them.  In some genera if you cross a 
diploid with a tetraploid you do get triploids, but in other plants 
there is triploid block, so any seeds that are produced from diploid x 
tetraploid crosses are tetraploids from a unreduced gamete from the 
diploid fertilized with the reduced gamete from the tertaploid - any 
triploids that form are aborted.  There are supposely two species of 
hosta that are tetraploids, so it should be possible to check for the 
presence of unreduced gametes.  Do you know if unreduced gametes are 
present in hostas, maybe at a very low level, and do you know if 
hostas have triploid block? 

There is, however, the question as to if tetraploid hostas have any 
value.  With a diploid chromosome count of 60 we can surmise that 
hostas are probably already amphidiploids.  Experiments on repeated 
doubling of chromosomes has shown that plants only tolerate so many 
chromosomes.  You don't find too many plants with chromosome counts of 
over 100.  With a chromosome count of 120 in a tet hosta there is a 
very high chance that all sorts of chromosome abnormalities will 
arise.  These abnormalities might not effect the plants ability to 
grow and survive, but it sure could mess up fertility in later 

Joe Halinar

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