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Sum and Substance explained ?

Some of you have, rightly so, questioned why S&S is a triploid
Gary Grosset suggested that S & S is possible an  F2 of elatior x 
yellow sieboldiana x hypoleuca. The problem is that crossing 
diploids will not give rise  to triploids or tetraploids but occasionally 
that happens All sieboldianas including the fully fertile Elatior have 
about 23.5 pg of DNA per nucleus, hypoleuca has 25.5
So lets assume first to cross hypoleuca x yellow sieboldiana -> an 
offspring with 24.5 pg. Crossing this with hypoleuca will give an F2 
with 25 pg  However if some nonreduced eggs are made the result 
will be 24.5 + 12.5 = 37.0 That is what I found so that is why I think 
it is triploid However I once measured Eagles nest and the result is 
36.5= triploid  for the edge and 51 for the centre making the centre 
containing 4.5 DNA sets, very weird!! If however S& S was a 
tetraploid Eagles nest would be with a tetraploid edge and a 
hexaploid centre However the assumption that S&S is a tetraploid 
is unlikley as it  would be impossible to find two parents that when 
doubled would give a large plant (only the small korean species 
have this amount of DNA ie 17-19 pg)
 Conclusion again as in my article: S&S is a triploid This explains 
too the weird results in its seedlings, that when S&S is selfed 
some are sterile (Garry) and the DNA amounts I found in 10 of its 
seedlings  So you can now (dis) prove this by just making these 
crosses. Actually I already have a hypoleuca x Love Pat...I must 
remember this year to cross it with Elatior. Maybe I get a blue S&S!
Ben J.M.Zonneveld
Clusius lab pobox 9505
2300 RA Leiden
The Netherlands
Fax: 31-71-5274999

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