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Re: Method for chromosome coloring

In a message dated 11/20/00 05:22:27 Eastern Standard Time, 
zonneveld@RULBIM.Leidenuniv.nl writes:

<< 6. Incubate in Feulgen reagent (Sigma) for 1-2 in the dark >>
Hi, Ben:

In step 6 above, you say to incubate for 1-2 what...  minutes, hours, or days?

"10. Look for cells in mitosis under the microscope and make 
drawings of all the different stages. If you cannot find any 
cells in mitosis, take a new root."  Please explain your statement to the not 
learned ones.  I hypothesize that the earlier cellular mitosis, the clearer 
it would be to count the chromosomes?  Is this the method one call, use for 
hosta chromosome counting?


Rich Horowitz
Stoughton MA
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