Alcohol will dissolve both anthrocyanin and cartenoids. So you will get
a mix of pigments. Crushed petals work better for extraction, as does
heating. Once pigments removed via alcohol extraction, place some
extraction liquid into a test tube (or equivalent narrow tube) with
some light clear oil (lamp oil will do) and shake vigorously and then
let sit. The oil soluble pigments will go into oil part, the water
soluble will remain in alcohol.
From: Linda Mann <email@example.com>
To: iris-photos <firstname.lastname@example.org>
Sent: Wed, Nov 20, 2013 6:41 am
Subject: [iris-photos] Re: HYB: seedling pigment?
After another day of soaking, plus heating the alcohol, the petal still
has some pigment, fading, more yellow looking now.
I was surprised too, but wonder if there was just so little anthocyanin
it didn't show in the soak water. It was one petal in about 2
tablespoons of alcohol, tho, so I would have thought <some> tint
show. The solution now has a pale yellowish pinkish cast. ???
soluble pink ;-) I guess something that soaked out has changed its
optical properties after sitting around for 2 or 3 days.
The petals on the live bloom also become more yellowbrown after they've
been open awhile.
Anthocyanins are also soluble in hot water, so maybe I'll boil up a
petal in just plain water, see what that does. Maybe the alcohol just
isn't working as well on this particular flower for some reason. I
started using alcohol because I thought/think that soaks out everything
but lycopene, & that's usually what I want to see if it's there or not,
but maybe hot water would be better on this one. Never did quite get
straight what pigment extraction diffs there are between hot water and
alcohol. All those different yellows and pinks and xanthophylls and
copigments and who knows whats.
<I hope you find an answer. I wouldn't have thought you'd have the
shown without some anthocyanin. That is the pigment that's soluble with
alcohol, isn't it? Maybe that bloom is just more yellow than it appears
to be in your photo, but when things get toast colored and start veering
into brown tones I've always thought that was due anthocyanin being
present. Maybe Chuck will see the post.>