From: christian foster <email@example.com_
(mailto:firstname.lastname@example.org) > Date: Tue, 15 Aug 2006
suspect that RHYTHM and or LITTLE FREAK may be diploid. Does anyone know
I was digging around in the archives trying to find any mention of sucessful
tetraploid X diploid crosses and stumbled upon mention of something called
It seems that the endosperm of a seed is created through a genetic process
which is seperate from the process that creates the embryo itself. This means
that a genetically viable embryo may be sabotaged in its quest for
germination by a genetically inviable endosperm. These seeds are characteristically
the ones that collapse, which is what all of my seeds from LF or R have done.
(ever so much more frustrating since they both set a pod if you look at them
Anyway, there is an embryo rescue technique which might be manageable in a
home environment... I think. Basically, you harvest really early, cut the pod
into little discs and lay the discs in a petrie dish with 'nutrient agar'.
From there I'm a bit cloudy. It seems like you would still have to proceed
with your cold stratification. In my refrigerator healthy seeds get all
moldy- and nutrient agar is intended to grow molds for kids science projects- so
how, I wonder, would I keep from just growing molds? There must be something
I missed. All the websites I looked at were either someone's doctoral
thesis, or for the fourth grade science fair.
i think I may put in a call to the county extension office....
Response from Dennis Hager, Society For Japanese Irises
Embryo and endosperm development are part of an interdependent reproductive
process. For fertilization to occur, the pollen grain must fertilize the egg
to form the embryo AND the endosperm. This is botany 101, but most of us
never took it or didn't get it.
As for embryo culture, it is a sterile process. It has been written up in
the AIS Bulletin at least 3 times, dating back to the early 1940's. Randolph
wrote the article. For over a decade, it was one of the most cited articles
published in the Bulletin.
If you would like to try embryo culture, I would recommend that you take a
course in plant tissue culture--or at least read a few books. Most
universities and many community colleges offer courses. If you insist on educating
yourself through the internet, there is a yahoo group for home tissue culture.
However, it is a marketing tool for the owner to promote weekend seminars on
home tissue culture.
Embryo culture skips the dormancy phase of germination, so there is no need
to stratify seed. In fact, fresh seed are easier to process. If you are going
to attempt embryo RESCUE (when there is no endosperm), then you have to
harvest the embryo and process it before it dies.
Also- On AIS Region 19 website, there is an AIS Bulletin Index database,
where you can search for old articles
Cedar Hill, MO
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