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Re: HYB:embryo rescue

Christian; many people in the Iris Society have done
embryo culture through the years. It is a great way of
ensuring success with crosses that would fail to
germinate. Growing the embryo is the most difficult
part. What one has to worry about is contamination and
having fungus or bacteria competing with the embryo on
the media. Excising the embryo under a controlled
airflow hood is ideal, but I know of a class of eight
year olds that made their own laminar flow hoods from
shoe boxes and had about a 60% success rate of growing
embryos. It may be like computers you have to find an
eight year old that can show you how to work it.

--- GormleyGreenery@aol.com wrote:

>  From: christian foster <_flatnflashy@yahoo.com_ 
> (mailto:flatnflashy@yahoo.com) >  Date: Tue, 15 Aug
> 2006
>  suspect that RHYTHM and or LITTLE FREAK may be
> diploid.   Does anyone know 
> for sure?
> I was digging around in  the archives trying to find
> any mention of sucessful 
> tetraploid X diploid  crosses and stumbled upon
> mention of something called 
> "embryo rescue".  
> It seems that the endosperm of a seed is created 
> through a genetic process 
> which is seperate from the process that creates the 
> embryo itself.  This means 
> that a genetically viable embryo may be  sabotaged
> in its quest for 
> germination by a genetically inviable  endosperm. 
> These seeds are characteristically 
> the ones that collapse,  which is what all of my
> seeds from LF or R have done.  
> (ever so much more  frustrating since they both set
> a pod if you look at them 
>  crosseyed.)
> Anyway, there is an embryo rescue  technique which
> might be manageable in a 
> home environment... I think.   Basically, you
> harvest really early, cut the pod 
> into little discs and lay the  discs in a petrie
> dish with 'nutrient agar'.  
> From there I'm a bit  cloudy.  It seems like you
> would still have to proceed 
> with your cold  stratification.  In my refrigerator
> healthy seeds get all 
> moldy- and  nutrient agar is intended to grow molds
> for kids science projects- so 
> how, I  wonder, would I keep from just growing
> molds?  There must be something 
> I  missed.  All the websites I looked at were either
> someone's doctoral  
> thesis, or for the fourth grade science fair.
> i think  I may put in a call to the county extension
> office....
> christian
> ky
> Response from Dennis Hager, Society For Japanese
> Irises
> Christian, 
> Embryo and endosperm  development are part of an
> interdependent reproductive 
> process. For  fertilization to occur, the pollen
> grain must fertilize the egg  
> to form the embryo AND the endosperm. This is botany
> 101, but most of  us 
> never took it or didn't get it.  
> As for embryo culture,  it is a sterile process. It
> has been written up in 
> the AIS Bulletin at least 3  times, dating back to
> the early 1940's. Randolph 
> wrote the  article. For over a decade, it was one of
> the most cited articles 
> published  in the Bulletin.  
> If you would like to  try embryo culture, I would
> recommend that you take a 
> course in plant tissue  culture--or at least read a
> few books. Most 
> universities and many community  colleges offer
> courses. If you insist on educating 
> yourself through the  internet, there is a yahoo
> group for home tissue culture. 
> However, it is a  marketing tool for the owner to
> promote weekend seminars on 
> home tissue  culture. 
> Embryo culture skips  the dormancy phase of
> germination, so there is no need 
> to stratify seed. In  fact, fresh seed are easier to
> process. If you are going 
> to attempt  embryo RESCUE (when there is no
> endosperm), then you have to 
> harvest the  embryo and process it before it dies.  
> Dennis  Hager 
> _hager@aredee.com_ (mailto:hager@aredee.com)  
> Also- On AIS Region 19  website, there is an AIS
> Bulletin Index database, 
> where you can search for old  articles 
> _http://www.jerseyiris.org/ais-index.htm_ 
> (http://www.jerseyiris.org/ais-index.htm)   
> Rita  Gormley
> Gormley Greenery
> Cedar Hill,  MO
> http://www.gormleygreenery.com/
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