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RE: Hybridizing PBF

I have found a cross of a PBF X green cross that produced 16 babies, none with PBF.  
This is Canadian Streaker X Designing Woman. CS is PBF (also varigated foliage, but these are completely independent factors)
Last year I presented mounting evidence that PBF is a partialy dominant rather then a completely dominant gene and that 3 or 4 PBF genes were needed for the PBF to show.  At that time I indicated that the last piece of comfirming evidence that was needed was a cross of a PBF parent to non-PBF   plant that produced no PBF offspring. This is the confirming evidence. The variagation present on thes seedlings certainly confirm CS parentage, and no other crosses with CS took that year. If PBF is dominant then half the offspring should be PBF. 16 plants is a large enough sample in this case to reduce the probability of this result by accident to be less then 1 in 100 cases. I didn't do the statistics, which would be a binomial probability function (like a coin toss) but it would likely give the probabilty of this as even much lower. (same as tossing a coin and getting 16 heads in a row.)

Thus PBF is partially dominant, showing with 3 or 4 PBF genes. Thus there are only two strengths of PBF. Other  variations on intensity would be due  to other factors. Ther is always the possibility that a plant with 2 PBF genes , in a cold spring aarea would show slight traces of PBF to a carefull observer.
Chuck Chapman, Guelph, Ontario, Canada. Zone 4/5

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