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Re: HYB: orange pigment extraction refresher?
  • Subject: Re: HYB: orange pigment extraction refresher?
  • From: Chuck Chapman <irischapman@aim.com>
  • Date: Sun, 01 May 2011 16:48:51 -0400

 To do pigment analysis thoroughly, should have a mashed up petal. First hot
water extraction. Filter mash though a coffee filter.

Next an oil extraction. Using colourless oil lamp oil works well. A little bit
of heat to aid extraction helps.

Now, after filtering, mix some alcohol with the oil extract, and shake.

Leave to settle out.  The lycopene remains in the oil layer, the other
cartenoids move to alcohol layer.

Use the purest alcohol you can. Some rubbing alcohols have a lot of water in

There can be some yellow water soluble pigments that wash out with the hot
water wash.

Some lycopene can dissolve in alcohol, but you get a good separation if you
use oil extraction, and then mix with alcohol.

Washing an orange  petal in warm alcohol will remove the carotenes leaving
most of the lycopene for observation, so you can also go that route.

Chuck Chapman

---- Original Message ----
From: Linda Mann <lmann@lock-net.com>
To: iris@hort.net
Sent: Sun, May 1, 2011 2:17 pm
Subject: [iris] HYB: orange pigment extraction refresher?

Chuck, if you have time, one more time re: pigment extractions, yellows,

First is hot water to get rid of anthocyanins, right?  Or does that also
removed one of the yellow pigments?

Second is alcohol, which definitely removes one of the yellows.

Third is oil (lamp kerosene is what I use, 'cause it's available) which
....uh, removes more yellow?  Ok, this is where I forget what that does.
After the kerosene soak. usually all I see left is the tangerine pigment
(either pink or red) left in the beard (if there is any), plus some yellow in
the thicker part of the petal near the beard.

Some results - skipping the water extraction, just using the alcohol step,

-  Mary Lou's pink HoM seedling: some yellow in the alcohol wash, pink left in
the petal

- Gold seedling from TEA LEAVES X GOLDEN PANTHER: yellow in the alcohol wash,
yellow in the petal, t in the beard.  I don't have GP any more to see what it

- HoM - yellow in alcohol, yellow in petal, no t in beard

- pale orangish seedling from GOLDEN APPLE X CRYSTAL GLITTERS: yellow in
alcohol wash, pink in petal, t beard.

If it were 'true' orange, would the alcohol wash look yellow, petal still look
sort of orange after alcohol wash?  i.e., pink plus yellow oil soluble?

Since the archive search engine doesn't seem to work for "and", maybe having
the words 'orange pigment extraction' all together will make it easier to find
the thread next time.

Linda Mann east TN USA zone 7

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