Hi all, TCing has no relation to nuclear implantation (as in Dolly). It takes meristematic tissue and grows it on a medium using chemical growth accellerators. The problem with TCing is the selection of the (merstem tissue) tissue in the first place and the culling of the plants after they are grown on to explant size. Selection has not been the best in some cases and neither has the culling so we get explants which may or may be "clones" of the mersitem tissue. WGS ----- Original Message ----- From: gw1944@vermontel.net Sent: Wednesday, February 20, 2002 12:06 PM To: hosta-open@mallorn.com Cc: PHOENIX_HOSTA_ROBIN@HOME.EASE.LSOFT.COM Subject: TC Questions I have got a couple of dumb as a stump questions on TC.
1. Clearly there is no F 1, F 2.. ...generation when it comes to TC. Is
there any hint, theory, or suggestion that one can get into trouble by
having the clone of a clone of a clone.......Is there anything to suggest
that by TCing a plant and then TCing that plant..... ad infinitum, that you
can get into trouble?
2. Does the actual age of the plant which is TCed make a difference on the
liners from that plant. I think that there was some problem with Dolly the
sheep in that respect. Are the animal kingdom and the plant kingdom
kissing cousins in this process?
3. Could Chick be TCed?
"Twas brillig, and the slithy toves did gyre and wimble in the wabe; and
mimsy were the borogoves." C.L. Dodgson
Glen Williams
20 Dewey St.
Springfield , Vermont
05156
Tel: 802-885-2839
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