Re: Method for chromosome coloring
- To: hosta-open@mallorn.com
- Subject: Re: Method for chromosome coloring
- From: H*@aol.com
- Date: Mon, 20 Nov 2000 07:36:35 EST
In a message dated 11/20/00 05:22:27 Eastern Standard Time,
zonneveld@RULBIM.Leidenuniv.nl writes:
<< 6. Incubate in Feulgen reagent (Sigma) for 1-2 in the dark >>
Hi, Ben:
In step 6 above, you say to incubate for 1-2 what... minutes, hours, or days?
"10. Look for cells in mitosis under the microscope and make
drawings of all the different stages. If you cannot find any
cells in mitosis, take a new root." Please explain your statement to the not
learned ones. I hypothesize that the earlier cellular mitosis, the clearer
it would be to count the chromosomes? Is this the method one call, use for
hosta chromosome counting?
Hostally,
Rich Horowitz
Stoughton MA
Z5
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