The coffee concentration was used in tissue culture to double up
haploid tissue from anthers of wheat. No records of anyone using it
with plants or seeds.
Chuck Chapman
-----Original Message-----
From: ChatOWhitehall <ChatOWhitehall@aol.com>
To: iris-species <iris-species@yahoogroups.com>
Sent: Sat, Jan 4, 2014 1:25 pm
Subject: Re: [iris-species] inducing tetraploidy
Â
I was reading something lately about coffee to induce. Not as simple s
it sounds. I don't recall the details, or where, but I think Chuck had
something to say about it, and may yet have things to share here. Not
to put him on the spot,Âreally.
Â
AMW
-----Original Message-----
From: Dennis Kramb <dkramb@badbear.com>
To: iris-species <iris-species@yahoogroups.com>
Sent: Sat, Jan 4, 2014 1:03 pm
Subject: [iris-species] inducing tetraploidy
 This topic has been discussed over the years,
but I never paid much attention because I knew I was never going to
mess with chemicals like colchicine at home. Lately I keep hearing of
less dangerous methods, and I'm planting seeds now, so I thought I'd
bring up the issue again.
How can I convert my iris seedlings to tetraploid? I dream of crossing
things like tetraploid Iris tridentata with tetraploid Iris fulva. I
played the waiting game and nobody created tetraploids for me :-) so I
guess I better do it myself. Or find a lab that can do it for me? Or
maybe SIGNA could sponsor some research students to do it in a proper
lab? I dunno....
Google searching is a bit overwhelming. But there seems to be a buzz
about less harmful chemicals such as Oryzalin. But I still get the
heebie jeebies about using mutagenic chemicals in my home. :-/
I know a daylily breeder nearby... maybe I could hire him to convert
some of my seeds. I also know a grad student at OSU... I'll bug him
too.
Dennis in Cincinnati
