Re: Re: inducing tetraploidy

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I have second generation plants from Iris foetidissima and Iris spuria maritima seedlings treated with Oryzalin. My problem is deciding whether plants are diploid or tetraploid. Second generation plants have the advantage that all the cells have the same "ploidy", while treated plants can have a mixture of cell types. My current idea is to photograph the chromosomes (which look a bit like tangled spaghetti even when they are organized for cell division) and see if I can see a difference between known diploids and my second generation plants. This winter I took some photos of  stained chromosomes in root tips of a diploid I. foetidissima, so I've starting on the project.

Ken Walker
Concord, CA USA

On 1/4/2014 1:20 PM, Paul Archer wrote:

 

I've been playing around with oryzalin based on a scientific article I found that had good results in daylily.  I calculated a rate using the Surflan concentration (I forget what the actual concentration and rate was) and the the smallest I could accurately measure was 0.5 ml using a diabetic syringe that I obtained from the pharmacy.  I had to ask for it specially (with sincerity and a smile) and explain what I was using it for.  0.5 ml in 1 Gallon distilled water and a drop of wetting agent such as spreader sticker or polysorbate 80.  You can make it ahead of time and store in the refridgerator for a while til you need it.

I haven't had much yet I wanted to use it on though so can't say how well it works for Iris.

-----Original Message-----
From: Chuck Chapman
Sent: Jan 4, 2014 4:09 PM
To: i*@yahoogroups.com
Subject: Re: [iris-species] Re: inducing tetraploidy

 

Much safer then colchicine. Use with same care as using household
chemicals such as bleach. Safer then bleach actually.

Chuck Chapman

-----Original Message-----
From: Sean Zera z*@umich.edu
To: iris-species i*@yahoogroups.com
Sent: Sat, Jan 4, 2014 2:27 pm
Subject: Re: [iris-species] Re: inducing tetraploidy

 
I've thought about trying the Oryzalin method, but I don't feel that
safe playing with chemicals either. The EPA says it's basically
nontoxic to humans when ingested, but is a probable carcinogen when
exposed chronically.

http://www.epa.gov/oppsrrd1/REDs/factsheets/0186fact.pdf

Sean Z

On Sat, Jan 4, 2014 at 2:05 PM, Dennis Kramb d*@badbear.com
wrote:
 
a non-iris friend sent me this link: 
http://members.tripod.com/h_syriacus/tetraploidy.htm

it's filled with many familiar names!  but those email addresses look a
bit out-of-date.  ;-)

oh, and the daylily guy already wrote back.  he told me "nope", he
doesn't mess with that stuff either.

Dennis in Cincinnati