It is far easier to just look at pollen under
microscope. Differences
are easily seen. Tetraploid pollen is larger.
Chuck Chapman
-----Original Message-----
From: Kenneth Walker k*@astound.net
To: iris-species i*@yahoogroups.com
Sent: Sun, Jan 5, 2014 2:21 pm
Subject: Re: [iris-species] Re: inducing tetraploidy
Â
I have second generation plants from Iris foetidissima
and Iris spuria maritima seedlings treated with Oryzalin.
My
problem is deciding whether plants are diploid or
tetraploid.
Second generation plants have the advantage that all the
cells
have the same "ploidy", while treated plants can have a
mixture of
cell types. My current idea is to photograph the
chromosomes
(which look a bit like tangled spaghetti even when they
are
organized for cell division) and see if I can see a
difference
between known diploids and my second generation plants.
This
winter I took some photos of stained chromosomes in root
tips of
a diploid I. foetidissima, so I've starting on the
project.
Ken Walker
Concord, CA USA
On 1/4/2014 1:20 PM, Paul Archer wrote:
Â
I've been playing around with oryzalin based on a
scientific article I found that had good results
in daylily. I calculated a rate using the Surflan
concentration (I forget what the actual
concentration and rate was) and the the smallest I could
accurately measure was 0.5 ml using a diabetic
syringe that I obtained from the
pharmacy. I had to ask for it
specially (with sincerity and a smile)
and explain what I was using it for.Â
0.5 ml in 1 Gallon distilled water
and a drop of wetting agent such
as spreader sticker or
polysorbate 80. You can make it
ahead of time and store in the
refridgerator for a while til
you need it.
I haven't had
much yet I wanted to use it on
though so can't
say how well it works for
Iris.
-----Original Message-----
From: Chuck Chapman
Sent: Jan 4, 2014 4:09 PM
To: i*@yahoogroups.com
Subject: Re: [iris-species] Re: inducing tetraploidy
Â
Much safer then colchicine. Use with
same care as using household
chemicals such as bleach. Safer then
bleach actually.
Chuck Chapman
-----Original Message-----
From: Sean Zera z*@umich.edu
To: iris-species
i*@yahoogroups.com
Sent: Sat, Jan 4, 2014 2:27 pm
Subject: Re: [iris-species] Re:
inducing tetraploidy
Â
I've thought about trying theÂOryzalin
method, but I don't feel that
safe playing with chemicals either. The
EPA says it's basically
nontoxic to humans when ingested, but
is a probable carcinogen when
exposed chronically.
http://www.epa.gov/oppsrrd1/REDs/factsheets/0186fact.pdf
Sean Z
On Sat, Jan 4, 2014 at 2:05 PM, Dennis
Kramb d*@badbear.com
wrote:
Â
a non-iris friend sent me this link:Â
http://members.tripod.com/h_syriacus/tetraploidy.htm
it's filled with many familiar names!Â
but those email addresses look a
bit out-of-date. ;-)
oh, and the daylily guy already wrote
back. he told me "nope", he
doesn't mess with that stuff either.
Dennis in Cincinnati
