SV: Embryo culture

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I found it in the 2008 Yearbook. WOW what a great set of photos!
You're absolutely right that details on the embryo extraction process
are what all the other articles fail to describe. One of them just has
a single sentence on it. LOL.

I just purchased a bunch of equipment (test tubes, forceps, scalpels,
funnel, etc.) to get me started but I haven't purchased any
agar/nutrient powders yet. Can you give me any suggestions? I'm mostly
doing spec-x crosses involving versicolor, and others with tectorum.

Oh by the way, if you grow yours in test tubes what size do you find
works best? I went with 1" x 10" since versicolor gets awfully tall
awfully fast.

Thanks, Ken!
Dennis in Cincinnati

On 5/9/2015 8:58 PM, kenww@astound.net [iris-species] wrote:
> I wrote a small article with photos on removing embryos
> from aril seeds back in 2009 for ASI. Because of the
> photos, the Word document is almost 3M, but it should be
> e-mailable if someone would like a copy.
>
> Ken
>
> On Sat, 9 May 2015 15:29:04 -0400
> "Dennis Kramb dkramb@badbear.com [iris-species]"
> <iris-species@yahoogroups.com> wrote:
>> I'm reading my old ASI yearbooks to learn the techniques
>> of the pros for
>> embryo rescue. it seems remarkably easy! I have some
>> TB seed and Juno
>> seed to practise with. I may try the forced/cutting
>> technique too, as
>> there are extremely good results reported with it as
>> well. I tried
>> scarifying some tridentata and tectorum seeds this
>> winter with 100% failure
>> rate. LOL. My tectorum seeds were surplus SIGNA seed
>> like you mentioned.
>>
>> Dennis in Cincy
>> On May 9, 2015 2:59 PM, "Walter Pickett
>> walterpickett@yahoo.com
>> [iris-species]"<iris-species@yahoogroups.com> wrote:
>>
>>>
>>> Yess, the kit is a luxury I couldn't, or didn't want to,
>>> afford.
>>> The MS medium is available from many chemical supply
>>> companies. It comes
>>> in bulk and in pre-measured packet for 1 liter distilled
>>> water, or
>>> deionized water. Some say they use tap water and I
>>> doubt they have any
>>> problems with it. For $1 per gallon for distilled, I've
>>> never tried tap
>>> water, but actually, while scientific journal articals
>>> always give exact
>>> recipes, there is a lot of room for improvizing. Think,
>>> how exact is the
>>> chemistry of the soil these iris grow in?
>>> Of iris, I have onlly done I. tectorum. The only reason
>>> is used the
>>> sterilized seeds and growing medium was the it wouldn't
>>> mold before
>>> germination. And I was praciticing for embryo culture
>>> with arils. I
>>> didn't want to risk the more rare arill seeds. I.
>>> tectorum is a fie plant,
>>> but it seeds easily and there are always leftover I.
>>> tectorum seeds after
>>> the sale, The Signa seed sale didn't even have any
>>> raail seeds this
>>> year. But of course the ASI has a seed sale every year.
>>>
>>>
>>>
>>> On Saturday, May 9, 2015 12:05 PM, "Dennis Kramb
>>> dkramb@badbear.com
>>> [iris-species]"<iris-species@yahoogroups.com> wrote:
>>>
>>>
>>>
>>> I am super annoyed at how few of my seeds have
>>> germinated so far this
>>> spring. I'm sick of the waiting game, so I'm gonna
>>> investigate this embryo
>>> rescue at home technique. I checked out the kitchen
>>> culture page and I had
>>> a little bit of sticker shock at the prices of their
>>> kits, so I'm gonna
>>> keep shopping around.
>>>
>>> Dennis in Cincinnati
>>>
>>>
>>> On Wed, Apr 22, 2015 at 4:33 PM, Walter Pickett
>>> walterpickett@yahoo.com
>>> [iris-species]<iris-species@yahoogroups.com> wrote:
>>>
>>>
>>>
>>> Sean.
>>> Embryo rescue isn't all that hard, depending on your
>>> eyesight. I have to
>>> use a diesecting scope now.
>>> There is a website called kitchen culture, or kitchen
>>> tissue culture, or
>>> such. Google will bring it up.
>>> In addition to the information on the site,, there is a
>>> link to an egroup
>>> of home tissue culture practicers, mostly amatures but
>>> including
>>> professionals and professors, etc.
>>> They are full of good information, and will answer
>>> questions.
>>> The chemicals are available from several chemical supply
>>> companies, the
>>> medium pre-measured for one liter of sterile distilled
>>> water. Our standard
>>> culture vessels are babyfood jars, which are not
>>> terribly expensive at your
>>> local food store full of babyfood, cheaper than buying
>>> babyfood jars
>>> online. The jars might be cheaper at a daycare if you
>>> can find one.
>>> I used to be quite active on the group, but I have been
>>> too busy this
>>> winter.
>>> Walter
>>>
>>>
>>>
>>> On Tuesday, April 14, 2015 8:15 PM, "Sean Zera
>>> zera@umich.edu
>>> [iris-species]"<iris-species@yahoogroups.com> wrote:
>>>
>>>
>>>
>>> Anita,
>>>
>>> It would be nice to be able to use that technique on
>>> hybrid seeds; I don't
>>> think it's too involved, but requires some basic plant
>>> micropropagation
>>> skills I don't have. :)
>>>
>>> As for the *koreana*, if it's really self-incompatible,
>>> I can just have
>>> someone mail me some pollen, or try wide crosses on it
>>> like Dennis.
>>>
>>> Sean Z
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>
>
> ------------------------------------
> Posted by:<kenww@astound.net>
> ------------------------------------
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