Re: HYB: Colchicine & the Triploid Route
- To: Multiple recipients of list <i*@rt66.com>
- Subject: Re: HYB: Colchicine & the Triploid Route
- From: c*@qntm.com (Chad Schroter)
- Date: Tue, 26 Aug 1997 10:57:48 -0600 (MDT)
I'm curious about the triploid method - is this the approach people will
be taking to increase the number of tetraploid Louisiana Iris ?
Chad Schroter in Los Gatos CA.
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Subject: HYB: Colchicine & the Triploid Route
Author: Sharon McAllister <73372.1745@compuserve.com> at SMTP
Date: 8/26/97 9:46 AM
1. Cross diploids having desired genes with the best available
tetraploids.
2. Cross the resultant triploids back to tetraploids. At this point=
,
fertility is limited so I save ANY seedling that produces offspring. I
also save any that are remarkable, in hopes they might later produce a
seedling or two. Don't expect any to be fully fertile, but watch for suc=
h
an anomaly because once-in-a-while an unreduced gamete will result in a
first-generation tetraploid.
3. Cross the second-generation seedlings back to tetraploids. Yes,
EVERY second-generation seedling. Not as hard as it sounds because there=
aren't many. Repeat the selection process with respect to both fertility=
and desired characteristics. In theory, about half should prove fully
fertile and half should be limitedly fertile. =
The third, and subsequent, generations will be predominantly fertile. =
Sharon McAllister
73372.1745@compuserve.com
