RE:Hyb: malvidin.and infrared Spectrocopy

A bit more research shows why infrared Spectrocopy is not used in analysing plant pigments. 

"Many compounds have a complex series of adsorptions spanning a range of wavelengths. This fingerprint region of the spectrum may be too difficult to analyse in detail, but because it is unique to a particular compound, we can identify an unknown compound by comparing its infrared spectrum to a large number of known spectra."

These spectra are more complicated with large molecules as in floral pigments.  In addition each compound must be purified  as having several compounds at once makes process more complicted. The best and easiest way to seperate pigments is the HPLC process itself which seperates the individual chemicals in a silica gel tube (or other similar filtering compound) under pressure. Once this is done there is no need for the infrared spectrophy. 

No wonder HPCL is the method of choice. Fast, effecive and accurate.  The equipment and suplies are expensive though and the computer program to run the whole costs about $1,600 .

Chuck Chapman

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