RE: HYB: freezing pollen question
iris@hort.net
  • Subject: RE: HYB: freezing pollen question
  • From: &* H* <c*@wi.rr.com>
  • Date: Fri, 25 Oct 2013 09:31:12 -0500

Hi Adam,
Would you take a picture of the vial's you use and send it?
Char

-----Original Message-----
From: owner-iris@hort.net [o*@hort.net] On Behalf Of Adam
Cordes
Sent: Thursday, October 24, 2013 7:46 PM
To: iris@hort.net
Subject: Re: [iris] HYB: freezing pollen question

Hi Linda, Bill, and others,

I'm glad you mentioned this topic, Linda.  I have an MTB guest seedling with
Aphylla in it's parentage that is coming into rebloom.  The hybridizer of it
sent it so I can use it in my median crosses, and highly recommended
freezing the pollen from this fall so I can use it in the spring, especially
if the plant decides it needs to wait another year before blooming.  

I currently have frozen pollen in snap-top vials that used to contain
diabetic test strips.  I have used some of them this summer in crosses,
mostly SDB pollens on TB plants for some intermediate seedlings to work
with.  I haven't yet completed my notes on the pod harvest to see if it
worked though.

I'd be interested in reading other success stories.  

Adam~

President, Mio Irisarians
Region 6
Zone 4 (and a half...)
Michigan

--------------------------------------------
On Wed, 10/23/13, Bill Chaney <billchaney@ymail.com> wrote:

 Subject: Re: [iris] HYB: freezing pollen question
 To: iris@hort.net
 Date: Wednesday, October 23, 2013, 12:16 AM
 
 I have great success freezing pollen
 and have several takes from pollen that has been frozen for  two years, and
I will try three year old pollen this next  year.
 
 Contrary to what many people say, I feel over drying is a  problem.  I take
fresh anthers with nice fluffy pollen  and place them into small glassine
envelopes and place them  directly into the freezer in a sealed container
(think small  Tupperware box).  I think it is best if the freezer is  NOT
frost-free.  I only place 6 to 9 anthers in an  envelope so that the number
of times the pollen is taken out  and returned to to freezer is minimized.
 
 Glassine envelopes will adsorb some moisture if there should  there be any
excess.  The other nice thing about these  envelopes is that you can recover
loose pollen from the  envelope with forceps.  If you what to try some, I
would be glad to and you a few if you request  off-list.  I buy them in bulk
from a company called  Bio-quip, but shipping small quants is expensive.
They  also have great forceps in their catalog.
 
 Please let me know if you have questions or need anything  else.
 
 Bill
 
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