Re: FW: FW: pumpkin pollen

GORDON,  I JUST WANTED TO THANK YOU FOR ALL THIS POLLINATION INFORMATION
                            MIKE IN ANCHORAGE
More pollen info from another professor.

Gordon Tanner
Maple Valley, WA

> ----------
> From:         Michael Orzolek[SMTP:mdo1@psu.edu]
> Sent:         Thursday, June 11, 1998 10:33 AM
> To:   Tanner, Gordon L
> Subject:      Re: FW: pumpkin pollen
>
> Gordon,
> When I was completing my doctoral program at the University of
> Maryland, my
> advisor was a tomato plant breeder.  I was responsible for making
> tomato
> crosses in the greenhouse and as you pointed out it was difficult to
> accurately predict female receptivity of pollen.  I would therefore
> collect
> pollen from specific varities we used fore crossing by tapping the
> anthers
> and collecting the falling pollen in gel capsules.  I would then store
> the
> pollen in capsules and place them in the refrigerator, temperature
> about
> 45°F for up to 7 days before the pollen became non-viable.  For
> pollination, I would emasculate (take the anthers off) the female
> parent
> flower and then coat the tip of the stylis with the pollen in the
> capsule
> (place the stylis - top of the stigma - into pollen until it was
> coated and
> visibly yellow).
> The success rate with this method in the greenhouse in the winter and
> early
> spring was between 25 and 40% pollinated fruit.  With pumpkins in the
> field, I would predict an 80 to 85% success rate.
> If you have additional questions, please contact me at your
> convenience.
> Good Luck!
> Mike Orzolek
>
> mdo1@psu.edu
> Michael D. Orzolek
> 203 Tyson Bldg.
> The Pennsylvania State University
> University Park, PA  16802
> Phone: (814) 863-2251
> Fax: (814) 863-6139
>
>
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