Cloning top lines.
- To: pumpkins@mallorn.com
- Subject: Cloning top lines.
- From: H* E* P*
- Date: Thu, 10 Jun 1999 04:45:37 -0700
- References:
pumpkins@mallorn.com wrote:
>
> I say, send that 567.5 seed to Harold Eddleman for the purpose of
> research (MR. Eddleman willing) and let him try to find out what makes it so
> special, it would be ashamed to lose the last few remaining 567.5 seeds
> without truly trying to find out what made them so great, (Elkskin)
>
In plant and animal breeding, there are some individuals which have
great reputations for passing great traits in matings. Earliglow
strawberry is an example. It has good taste, resistance to a few
diseases and passes these to offspring with greater than average
success.
If the Mombert 567.5 is such a fruit, there is no guarantee that a
given seed from that fruit will do so.
Nonetheless, I feel we should look into establishing some lines from
such outstanding seeds in vitro. It is likely that some seeds too old to
germinate could be rescued by in vitro efforts.
I am planning to try some of this. The first step would be to get lots
of experience with "discard grade" seeds. The main problem is getting an
explant growing in vitro free of molds and bacteria. Then comes the
problem of multiplying (usually easy) and getting them rooted in the
greenhouse (often impossible). Since AG makes lots of roots on the vine,
it will probably be fairly easy to get the microcuttings from MP rooted
in the Greenhouse.
More practical would be to clone the orginal plant. If you grow an
outstanding fruit this year, why not attempt to keep a cutting over
winter by rooting it in a pot?
--
Harold Eddleman Ph.D. Microbiologist. i*@disknet.com
Location: Palmyra IN USA; 36 kilometers west of Louisville, Kentucky
http://www.disknet.com/indiana_biolab/pk.htm
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