Re: Flat Vines
- To: pumpkins@mallorn.com
- Subject: Re: Flat Vines
- From: H* E* P*
- Date: Sat, 19 Jun 1999 04:00:31 -0700
- References:
pumpkins@mallorn.com wrote:
>
> Harold & List,
>
> Your recent tome gives credence to a question I've
> had nagging in the back of my head since
> discovering flat vine on my 1092Burke. These genes
> which you mentioned are
> present in the seed, which we germinate in
> artificial conditions. Could the constant
> temperatures above say
> 85 degrees F cause gene mutation
HE: Temperature would not cause a mutation. Mutation rates are
usually only 1 per 100,000 to 10,000,000 individuals.
> or a higher instance of penetrance of flat vine.
HE: Temperature could be important. In a grain beetle, I found
only few percent of beetles show the 'scar' mutation at normal
temperatures, but nearly 100% penetrance at 98F.
>
> On my 1092 Burke I used slightly higher temps in
> my growth chamber during germination trials (87.5
> degrees F).
> I had two seed, only one germinated. Last year I
> used
> 84.5 degrees and all germinated.
HE: I did not use an incubator; I used hot water bottles.
I got the feeling that temperatures of 92F are too hot for
good germination. I have some nice incubators. Once I grow
some seed, I can check effects of various temperatures.
> --
> Greg Schraiber
> Machesney Park, IL
> --
Harold Eddleman Ph.D. Microbiologist. i*@disknet.com
Location: Palmyra IN USA; 36 kilometers west of Louisville, Kentucky
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