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Re: alocasia DNA sample 1

  • Subject: Re: alocasia DNA sample 1
  • From: "Julius Boos" <ju-bo@msn.com>
  • Date: Mon, 4 Mar 2002 15:25:18 -0600 (CST)

----- Original Message -----
From: zonneveld <zonneveld@rulbim.leidenuniv.nl>
To: Multiple recipients of list AROID-L <aroid-l@mobot.org>
Sent: Monday, March 04, 2002 10:47 AM
Subject: alocasia DNA sample 1

Dear Ben,

I am NOT good at Alocasias, but in Deni Bown`s second edition of her book
'Aroids--Plants of the Arum Family', Alocasia 'Tigrina superba' is
considered but a 'cultivar' of Alocasia zebrina (pg 184) .


>>Denis of Silver Krome Gardens Homestead Florida was so kind to
send me leaf a samples of 11 Alocasias. I measured their DNA and
got t some interesting results I will treat them in small groups for
you so as to invite your comment as I dont know anything about
Alocasias .
General: A sample of leaf was chopped with the standard Agave
americana of 15.9 pg Nuclei were""colored"" with propidium iodide
and measured one by one in a flow cytometer. About 5000 to
10.000 nuclei were measured and the values in Alocasia range
from 10 -22 picogram Furhter I noted that three samples had very
dark suspensions.
I found 11.2 picogram per nucleus for Alocasia tigrina superba and
11.6 pg for A zebrina reticulata . These values are too close for
comfort They might be closely related species or Could they be the
same species?
Ben J.M.Zonneveld
Clusius lab pobox 9505
2300 RA Leiden
The Netherlands
Fax: 31-71-5274999

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