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Re: Re: Re: GA3 in seed germination

  • Subject: Re: Re: Re: GA3 in seed germination
  • From: "Nyles" <metopium@hotmail.com>
  • Date: Wed, 30 May 2001 08:32:43 -0500 (CDT)


I've used KOH and GA together without adjusting the Ph on new shoot and 
flower growth with no problems.
Once you have the Potassium salt (KOH+GA), it is more potent than "plain" GA 
(according to literature) and freely soluble in water.
That's how I'd personally go based on past experience.

Having said that, I am not saying the other method wouldn't work or that 
this method will get you the outcome you desire.
I've just had big problems with ethanol (however small)+water and GA killing 
tissue cultures.

If in doubt use both. I'd be curious how it turns out, either way.

Hope I've helped more than confused!


>From: mburack@mindspring.com
>Reply-To: aroid-l@mobot.org
>To: Multiple recipients of list AROID-L <aroid-l@mobot.org>
>Subject: Re: Re: Re: GA3 in seed germination
>Date: Tue, 29 May 2001 20:59:20 -0500 (CDT)
>Let me beat the horse one more time,
>If it cant be done, what do I do.....? Put a little in water, add some 
>vodka or KOA and mix it up?
>Doesnt the introduction of these other chemicals alter the effects of the 
>GA itself?
>I hate to keep asking these questions, but I have the stuff, and I just 
>want to give it a go.  I would be willing to mix it with anything if it 
>will work.
>As a matter of fact, you guys are talking about mixing 1 gm / liter, and I 
>was under the impression (based on something that I read) that the easiest 
>way to go was to dip a toothpick about an 1/8 of an inch into the powder, 
>dissolve (assuming that it could be done) into a cup of water and soaking 
>the seeds in it for 24 hours.... Does this sound right?  I figure if I am 
>going to do it, I would like to do it right.
>Also Niles, there has been the talk about the pH, if I were to go the "KOA" 
>route, how would I "right" the pH? Or is alcohol the easier way? Somebody 
>just has to tell me what to do :)
>aroid-l@mobot.org wrote:
> > Not to beat a dead horse.
>In my personal experience, I've never gotten GA to disolve in any amount of
>water, certainly not at 1gm/liter.
>I'm not saying it can't be done, just that I haven't been able to do it.
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