Re: Re: Re: GA3 in seed germination

Hi!

I've used KOH and GA together without adjusting the Ph on new shoot and 
flower growth with no problems.
Once you have the Potassium salt (KOH+GA), it is more potent than "plain" GA 
(according to literature) and freely soluble in water.
That's how I'd personally go based on past experience.

Having said that, I am not saying the other method wouldn't work or that 
this method will get you the outcome you desire.
I've just had big problems with ethanol (however small)+water and GA killing 
tissue cultures.

If in doubt use both. I'd be curious how it turns out, either way.

Hope I've helped more than confused!

Nyles




>From: mburack@mindspring.com
>Reply-To: aroid-l@mobot.org
>To: Multiple recipients of list AROID-L <aroid-l@mobot.org>
>Subject: Re: Re: Re: GA3 in seed germination
>Date: Tue, 29 May 2001 20:59:20 -0500 (CDT)
>
>Let me beat the horse one more time,
>
>If it cant be done, what do I do.....? Put a little in water, add some 
>vodka or KOA and mix it up?
>
>Doesnt the introduction of these other chemicals alter the effects of the 
>GA itself?
>
>I hate to keep asking these questions, but I have the stuff, and I just 
>want to give it a go.  I would be willing to mix it with anything if it 
>will work.
>
>As a matter of fact, you guys are talking about mixing 1 gm / liter, and I 
>was under the impression (based on something that I read) that the easiest 
>way to go was to dip a toothpick about an 1/8 of an inch into the powder, 
>dissolve (assuming that it could be done) into a cup of water and soaking 
>the seeds in it for 24 hours.... Does this sound right?  I figure if I am 
>going to do it, I would like to do it right.
>
>Also Niles, there has been the talk about the pH, if I were to go the "KOA" 
>route, how would I "right" the pH? Or is alcohol the easier way? Somebody 
>just has to tell me what to do :)
>
>
>
>aroid-l@mobot.org wrote:
> > Not to beat a dead horse.
>
>In my personal experience, I've never gotten GA to disolve in any amount of
>water, certainly not at 1gm/liter.
>I'm not saying it can't be done, just that I haven't been able to do it.
>
>Nyles
>
>
>
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