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Re: [aroid-l] A. Titanum cloned

  • Subject: Re: [aroid-l] A. Titanum cloned
  • From: "WEAVER,BILL (HP-USA,ex1)" bill.weaver@hp.com
  • Date: Fri, 19 Sep 2003 15:02:31 -0700

I used just a leaflet one of the little (4" X 8") oval components that make 
up the entire leaf. I just took one that came to a nice point with very
little 
leaf tissue on either side of the mid-rib where I cut it off.

Bill

-----Original Message-----
From: Scott Taylor [mailto:staylor@brevardparks.com]
Sent: Thursday, September 18, 2003 2:02 PM
To: aroid-l@lists.ncsu.edu
Subject: Re: [aroid-l] A. Titanum cloned


Just so I am clear on this cloning technique: does one use the entire leaf,
or trim the lateral material away from the midvein?  And how much of the
petiole is retained?
Thanks
dst

D. Scott Taylor, Ph.D.
Brevard County Environmentally Endangered Lands (EEL) Program
Central Region Land Manager
5560 North US Highway 1
Melbourne, FL 32940
tel: 321.255.4466
FAX: 321.255.4499
email: staylor@brevardparks.com


-----Original Message-----
From: aroid-l-owner@lists.ncsu.edu
[mailto:aroid-l-owner@lists.ncsu.edu]On Behalf Of Douglas Ewing
Sent: Thursday, September 18, 2003 11:36 AM
To: 'aroid-l posting'
Subject: Re: [aroid-l] A. Titanum cloned


I too have been cloning titanum from leaf cuttings. We differ from Bill's
procedure slightly:

	I use a 12" portion of the leaf with the mid-vein. I have not
experimented with smaller cuttings.

	We apply no hormone. ( this is something I have avoided with all
leaf cuttings in the past, as I was under the impression that auxin would
promote root formation, but that it inhibits formation of shoots. Since we
want both to happen with a  leaf cutting, we do not apply.  Tubers are
shoots, perhaps this explains the difference in the gel vs. powder
results?)

	We stick the cutting into a 8cm cube of rockwool, water, and
encapsulate the entire affair in a terrarium made from 2  2liter
pop-bottles. This creates almost total humidity, and cutting do not wilt.
If/when fungus appears on the cutting, we dust with cinnamon. Tuber
formation
takes several months, then we wean from humidity by utilizing a pop-bottle
with the bottom removed and the cap off, this allows a bit of air exchange
for a few days , then the cutting is removed from the structure, potted up
and placed on the open bench. Typically the orig. leaf tissue dies,
followed by new leaf emergence. We also see multiple leaf emergence, which
I am assuming will result in multiple tubers.

	I have not tried other sp. of this genus, but the fact that
several species produce tubercles on the leaf naturally,( A. bulbifer)
makes me think that all sp. might respond to this treatment.


Doug
_________________/\/\/\______________________

Doug Ewing, Greenhouse Manager (206) 543-0436
Department of Biology
University of Washington
Box 351800
Seattle, WA   98195-1800









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