Seeds would work well. Easier to get across border.
Not sure how much material I'll need. A leaf sample may be enough,
but fresh material is always best. Haven't done this before so may
need some practice. Getting seeds into Greenhouse at University is
easy. They frown on bring plants in, need quarantine and space for
that is not always available.
I may have to get any plants growing in my own greenhouse and take them
over to University as a group for the quarantine period.
There is a number of the necessary gene sequences in gene databank.
I'll need to check what clones they have there. And what sequences are
available. Need same gene or genes to compare. I think I remember that
they have a number from Louisiana, perhaps one from Texas and at least
one from Florida. None from Ohio, so that will be one needed
particularly.
The more samples, the better the phylogenetic tree, but also more
difficult to sort out best fit.
Chuck Chapman
-----Original Message-----
From: Rodney Barton rbartontx@yahoo.com [iris-species]
<iris-species@yahoogroups.com>
To: iris-species <iris-species@yahoogroups.com>
Sent: Wed, Apr 29, 2015 8:44 am
Subject: Re: [iris-species] Re:Iris brevicaulis Ontario
Â
I do know the general area where this one was collected.
Will you be isolating DNA from leaves? I'm wondering if it would be
easier or more expedient for everyone to send you leaves rather than
rhizomes.
R
From: "Chuck Chapman irischapman@aim.com [iris-species]"
<iris-species@yahoogroups.com>
To: iris-species@yahoogroups.com
Sent: Tuesday, April 28, 2015 10:12 PM
Subject: Re: [iris-species] Re:Iris brevicaulis Ontario
 Yes I would. With any plant I would need to
know it's collection site, as near as possible. If I have a number of
different localities a mini phylogeny could be constructed and it
then be possible to see if Ont brevicaulis is a relic , at root or a
branch off the main population. It would provide interesting data
when all put together. Data on a number of brevicaulis clones are
already in gene back, and can be accessed.Chuck Chapman-----Original
Message----- From: Rodney Barton rbartontx@yahoo.com [iris-species]
<iris-species@yahoogroups.com>To: iris-species
<iris-species@yahoogroups.com>Sent: Tue, Apr 28, 2015 9:09
pmSubject: Re: [iris-species] Re:Iris brevicaulis Ontario ÂI have what
I believe is a brevicaulis from Texas that I could contribute if it
would be of interest.ÂRod From: "Chuck Chapman
irischapman@aim.com [iris-species]" <
iris-species@yahoogroups.com> To: iris-species@yahoogroups.com Sent:
Tuesday, April 28, 2015 12:50 PM Subject: RE: [iris-species] Re:Iris
brevicaulis Ontario That would be great. Do you
have the means to get it here? Seeds may work better.What are the
possibilities of samples of the Ohio plant? A phylogenetic tree of
brevicaulis would be neat. It would defiantly be able to show
migration route, and genetic drift.Chuck Chapman-----Original
Message----- From: 'Patrick O'Connor' pfoconnor@cox.net [iris-species]
<iris-species@yahoogroups.com>To: iris-species
<iris-species@yahoogroups.com>Sent: Tue, Apr 28, 2015 1:09
pmSubject: RE: [iris-species] Re:Iris brevicaulis Ontario ÂChuck,ÂI can
certainly send you I. brevicaulis from Louisiana.ÂPatrickÂFrom:
iris-species@yahoogroups.com [mailto:iris-species@yahoogroups.com]Sent:
Tuesday, April 28, 2015 9:19 AMTo: iris-species@yahoo
groups.comSubject: RE: [iris-species] Re:Iris brevicaulis OntarioÂÂ I'm
hoping to be able to get a sample and do a genetic comparison witha
brevicaulis from Lousiana. Should prove to be interesting. I'm suretime
separation from Louisiana brevicaulis will have produceddifferences. So
different enough to be a new species?Colour and form same as a number
of colour variations of versicolor,but signal bristles much larger.
Haven't located a picture offlowering stem yet. It may still prove to
be some sort of hybrid.Hoping tolocate herbarium sampleChuck
Chapman-----Original Message-----From: 'Patrick O'Connor'
pfoconnor@cox.net [iris-species]<iris-species@yahoogroups.com>To:
iris-species <iris-species@yahoogroups.com>Sent: Tue, Apr 28,
2015 7:34 amSubject: RE: [iris-species] Re:Iris brevicaulis OntarioÂThe
color of this Ontario brevicaulis is interesting. It seems to havemuch
more red in it than brevicaulis I have seen from the South, whichare
almost all in the blue and lavender range. There was a
possiblebrevicaulis documented in Northen Ohio that seems to have that
samecolor
trait.Âhttp://www.ohionatureblog.com/2009/06/its-always-nice-to-find-what
-youre.html Perhaps it is a regional variation, and perhaps there has
been somepast introgression with I. fulva.ÂPatrickÂ
;From:iris-species@yahoogr oups.com
[mailto:iris-species@yahoogroups.com]Sent: Monday, April 27, 2015 6:25
PMTo: iris-species@yahoogroups.comSubject: [iris-species] Re:Iris
brevicaulis Ontario [1 Attachment]ÂÂ Being doing some research. Found
original article. It was inUniversity's archives , so will be a few
days for them to dig it out.Here is a photo courtesy of P. Allen
Woodliffe, who was involved inMinistry of Natural Resources. Apparently
there were originally about10 populations, but may only be two left in
conservation areas. R estreplaced by development. Located on Pele
Island, south of Point Pelein Ontario. This is the most southerly part
of Ontario, same latitudeas North California.Close up of flower does
show the signal bristles. But I also saw someon by blowups of I
versicolour, but quite a bit sma ller. Currently nophotos of stem.
Still tracking down a herbarium specimen.I just may be able to get a
plant. I'll be working on it. The geneticsof this plant will have an
interesting tale to tell. If a truebrevicalus, it may be part of a
relic population with information onmigration route of the Louisiana
iris..Allen gave permission for his photos to be posted on SIGNA, if
thereis an interest.Chuck Chapman
