Oryzalin

 

Dear Dennis,
 
Not being a scientific person myself, I am curious as to whether seedling size will affect the ploidy conversion of the seedlings.  That is to say,  and how you will know wether you will achieve 100% conversion.
 
Joyce Miller, in Gresham, Oregon
 
From: i*@yahoogroups.com
Sent: Monday, June 23, 2014 5:07 PM
To: i*@yahoogroups.com
Subject: [iris-species] Oryzalin [1 Attachment]
 
 

With 15 seeds out of 30 germinated I decided it was time to subject my Iris tectorum seedlings to their first Oryzalin bath.  The ultimate goal is to create a tetraploid Iris tectorum to hybridize with TBs.

My technique may cause the scientists of this group to cringe, but this is how I decided to try it.  The proper way is with test tubes & agar & carefully measured solutions.  That intimidated me so I went with this...

With the variation in seedling sizes I might succeed with converting a few.  Oryzalin, unlike colchicine. shouldn't kill any of them.

I promised Rodney an article in a year or two... but for now I'll post a random pic or two as things progress.

Dennis in Cincinnati

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