Re: Oryzalin

 

I'm not planning to do flow cytometry or anything. I'll just go by appearances. Tetraploids should be slightly bigger, with thicker stems & flower parts. Even partially converted plants should be able to produce *some* fully converted pollen, which is enough to get started.

I have plenty more seeds to experiment with. My original batch size of 30 might end up being too small. I know that some other people are working on this same activity, and they're a year or two ahead of me. One of us is bound to succeed. Frankly I'm surprised this wasn't achieved 25-50 years ago.

For me the possibilities of crossing a tetraploid tectorum with exotic looking arils & arilbreds is tantalizing. I crossed Satan's Mistress with a diploid tectorum last year, but I'm still waiting for the seeds to germinate. I made many more crosses like that this year & I'm waiting for the pods to mature. I hope they're not balloons. I'll probably apply the Oryzalin treatment to those as well.

Dennis in Cincinnati




On Wed, Jun 25, 2014 at 1:38 PM, 'Joyce Miller' M*@comcast.net [iris-species] <i*@yahoogroups.com> wrote:


Dear Dennis,
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Not being a scientific person myself, I am curious as to whether seedling size will affect the ploidy conversion of the seedlings. That is to say, and how you will know wether you will achieve 100% conversion.
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Joyce Miller, in Gresham, Oregon
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From: i*@yahoogroups.com
Sent: Monday, June 23, 2014 5:07 PM
To: i*@yahoogroups.com
Subject: [iris-species] Oryzalin [1 Attachment]
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With 15 seeds out of 30 germinated I decided it was time to subject my Iris tectorum seedlings to their first Oryzalin bath. The ultimate goal is to create a tetraploid Iris tectorum to hybridize with TBs.

My technique may cause the scientists of this group to cringe, but this is how I decided to try it. The proper way is with test tubes & agar & carefully measured solutions. That intimidated me so I went with this...

With the variation in seedling sizes I might succeed with converting a few. Oryzalin, unlike colchicine. shouldn't kill any of them.

I promised Rodney an article in a year or two... but for now I'll post a random pic or two as things progress.

Dennis in Cincinnati




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