Re: Re: Iris abicans/questions and blather

I have Simonet's method detailed here that I could copy for you. Its faily elaborate. I have some OCR software that hasn't been installed and could scan it for you. There is  likely is a more up to date technique, but any I've seen seem to be faily similar.

Another project, that might be easier would be the Anthocyanin Vascular Intrusions in flower petals. I can send you a copy of my article on it detailing it as being the likely factor the takes us from blue and blue-violet pigmentation into the purple and black. This process would involve peeling off the top layer of cells on the flower and examining it under the microscope.

If you are at all interested let me know and I can pinpoint what needs to be looked at. Right now I have been gathering the supplies and equipment to do paper and  thin-layer chromotophagry and photo-spectromic  anysis of pigments. Just about all I need right now is the chemicals and I have a supplier lined up. Just have to figure out which ones will give me best differentiation of pigments.

Chuck Chapman 

oneofcultivars@aol.com wrote:

>In a message dated 3/26/2005 12:23:47 PM Central Standard Time, 
>irischapman@netscape.net writes:
>
>
>> Perhaps someone else could look over this document and comment.
>> 
>
>I do have a microscope. I do have albicans. Given time, proper supplies, and 
>some needed education, I will count them at some point. I've never done this 
>before.
>
>Prior to attempting the counting process, I hope to purchase or construct an 
>apparatus to attach my present camera to the microscope and perform the counts 
>from the resulting photographs. At this point I do not even know if the lens 
>focal length of my present camera will allow this to be done. Pressed for time 
>otherwise now like everyone else. I'm Always slow to get cranked here. My 
>wife says a procrastinator while kinder people say nonchalant. Me? Pure lazy. No! 
>Poor and pure lazy while trying to absorb too much by osmosis!
>
>For now, how many counts yielding the same number of chromosomes would be 
>considered reasonable to insure accuracy?
>
>Should healthy root tips collected/examined any time of the year produce 
>countable chromosomes?
>
>Any help/advice/speculation appreciated.
>
>Smiles,
>Bill Burleson
>
>
>
>

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