Re: Re: Iris abicans/questions and blather

Not any experience in counting Iris, but one thing comes to mind.  Counts made on meiotic material are usually much more accurate than from somatic tissue (particularly from root tips).  Root tips are notorious for having irregularities, due usually apparently to the rapid growth with tends to lead to mistakes.  A common mistake is the failure of a cell to split after the chromosomes replicate, leading to false polyploid counts.  On the other hand, some groups are apparently less prone to these problems than others, and from what I recall (somebody correct me if I'm wrong), root tips seem to be the standard for Iris counts.
----- Original Message -----
Sent: Saturday, March 26, 2005 3:02 PM
Subject: Re: [iris-species] Re: Iris abicans/questions and blather

In a message dated 3/26/2005 12:23:47 PM Central Standard Time, irischapman@netscape.net writes:


Perhaps someone else could look over this document and comment.


I do have a microscope. I do have albicans. Given time, proper supplies, and some needed education, I will count them at some point. I've never done this before.

Prior to attempting the counting process, I hope to purchase or construct an apparatus to attach my present camera to the microscope and perform the counts from the resulting photographs. At this point I do not even know if the lens focal length of my present camera will allow this to be done. Pressed for time otherwise now like everyone else. I'm Always slow to get cranked here. My wife says a procrastinator while kinder people say nonchalant. Me? Pure lazy. No! Poor and pure lazy while trying to absorb too much by osmosis!

For now, how many counts yielding the same number of chromosomes would be considered reasonable to insure accuracy?

Should healthy root tips collected/examined any time of the year produce countable chromosomes?

Any help/advice/speculation appreciated.

Smiles,
Bill Burleson





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