RE: Re: Iris abicans/questions and blather

The parafin method works well for chromosome and cell structure studies, not sure how it would work for pigments views. Probably not based on what the picure of the cell with pigment showed, as all the preperation involved in this would likely remove the anthocyanin. Also need a microtome to slice off a sample and all the chemicals and lab equipment for the prep work. I need to get over to the university to get a full copy of AVI paper which would likely have their method detailed or referenced. Without a student parking pass you have to park about a half mile from the library which always make this a much longer chore then it need to be. 
The stain shows up the structures after natural colours are removed by the prep work.

Chuck Chapman

"skyland 1" <lmmunro@hotmail.com> wrote:

>When I was at college in histoilogy class,way long time ago,  we stained our 
>tissue, then embedded in wax, then sliced into paper thin wafers to see the 
>internal structures of cells under the microscope. Would this be of any 
>help? While I don't remember the stains used, there are several standard 
>ones. I remember doing fruit fly chromosomes that way.
>Laetitia
>
>>From: "irischap" <irischapman@netscape.net>
>>Reply-To: iris-species@yahoogroups.com
>>To: iris-species@yahoogroups.com
>>Subject: [iris-species] Re: Iris abicans/questions and blather
>>Date: Tue, 29 Mar 2005 15:11:01 -0000
>>
>>
>>Hope you had some succes with albicans.
>>Yesterday I was able to peel off a top layer from a primula flower. I
>>sliced across the petal with an exacto knife held at an angle of about
>>30 degrees in a start of slicing off a section. The top layer was then
>>caught and it peeled nicely.
>>Under the kids microscope I was able to see the cells and some
>>internal structures but not in great detail as resolution and optics
>>lacked somewhat. Did clearly have just one layer of cells.
>>
>>Chuck Chapman
>>
>>
>>
>
>
>

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