Ken,
This sounds really interesting. Would you consider writing up the
process in detail, so we could publish it in the SIGNA bulletin?
Ken
Kenneth Walker wrote:
>
> I decided to try my hand at chromosome counting a couple years ago.
> After a long search I found a company that would sell aceto-carmine
> solution to an individual (the acetic acid component is 9 times stronger
> than vinegar, so the liquid and fumes are a bit caustic - most suppliers
> don't want the liability). Event then the process proved more difficult
> than I had hoped. After a number of attempts, yesterday I managed to get
> a picture of one cell where the chromosomes were separated enough and I
> managed to get the microscope focused just right so that I can count 30
> chromosomes. The photo is attached; the scale is about 16 pixels per
> micron. I had to look at huge number cells to get that one photo.
>
> I grew the Iris pumila from seeds, SIGNA 03A011. I. pumila comes in 30 &
> 32 chromosome varieties where the 32 variant has 4 sets of 8
> chromosomes. I assume the 30 chromosome version has lost a pair of
> chromosomes at some point.
>
> Ken Walker
> Concord, CA
>
>
>
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>
