Iris pumila, a different photo

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I decided to try my hand at chromosome counting a couple years ago.
After a long search I found a company that would sell aceto-carmine
solution to an individual (the acetic acid component is 9 times stronger
than vinegar, so the liquid and fumes are a bit caustic - most suppliers
don't want the liability). Event then the process proved more difficult
than I had hoped. After a number of attempts, yesterday I managed to get
a picture of one cell where the chromosomes were separated enough and I
managed to get the microscope focused just right so that I can count 30
chromosomes. The photo is attached; the scale is about 16 pixels per
micron. I had to look at huge number cells to get that one photo.

I grew the Iris pumila from seeds, SIGNA 03A011. I. pumila comes in 30 &
32 chromosome varieties where the 32 variant has 4 sets of 8
chromosomes. I assume the 30 chromosome version has lost a pair of
chromosomes at some point.

Ken Walker
Concord, CA