Re: Re:Auto-tetraploid versus amphidiploid was Hyb spots
iris@hort.net
  • Subject: Re: Re:Auto-tetraploid versus amphidiploid was Hyb spots
  • From: C* C* <i*@aim.com>
  • Date: Sun, 3 Mar 2013 10:16:16 -0500 (EST)
I'm, sure you were using the modern method of arresting development using colchicine. Light microscopes haven't improved all that much in past 70 or so years. But if you have what you want on one slide ( which is what happens using colchicine) you don't need to keep changing slides. Once you have located the cells you are looking at, you can switch to oil lens. not that much of a problem . When not fixed with colchicine, you need lots and lots of samples to get what you want. Constantly changes slides, Hours and hours of searching to get cells at exactly the right stage. The extra time going back and forth from slides, just not worth the time,added to an already extremely time consuming procedure. What you do if change focus, looking at slide in layers. by that you can get a good view. What I can do at lower focus, is take pictures at several different levels of focus, then put photos together in merge, using photoshop. This gives extremely good photos.
The hand drawing, substitutes for the many layers of focusing required. Not needed now when you can merge photos.
When Randolph took a picture, for his articles, it was at one layer of focus, so it just didn't capture the detail he built up in had when able to see all the different layers. Focal point under light microscope is very limited. 

Chuck Chapman

-----Original Message-----
From: Linda Mann <lmann@lock-net.com>
To: iris <iris@hort.net>
Sent: Sun, Mar 3, 2013 9:55 am
Subject: Re: [iris] Re:Auto-tetraploid versus amphidiploid was Hyb spots

My first two jobs - the first for 6 months looking at fern sporangia
(University of Tennessee), the second for a year looking at mouse
chromosomes (Biology Division, Oak Ridge Natnl Lab).  So long ago, I
barely remember what we were doing, but I know the microscopes we had at 
the Lab was orders of magnitude better, easier to get good counts &
document abnormalities.  hmm.. why in the world were we drawing by hand
in the 1968? Did that predate cameras attached to microscopes?? I feel 
like such a fossil!

 On 3/3/2013 9:05 AM, Chuck Chapman wrote:
By using colchicine, cells are arrested at exactly the right time 
for a

good observation.


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