Re: HYB: Abbey Road pigments (was on photos)

1)The red veins rules out it being a glaciata.

2)The water extraction proves that there is a water based yellow pigment, probably a flavanoid. 
The oil base pigment is either an emulsion, as this oil and alcohol don't fully seperate (speculation),  or an oil based pigment but not likely a cartenoid 

Thanks loads.

Chuck Chapman


>Date: Wed, 11 May 2005 21:21:28 -0400
>From: Linda Mann <lmann@volfirst.net>
>Subject: [iris] Re: HYB: Abbey Road pigments (was on photos)
>
>Thanks Neil - she definitely has some red looking veins.  Not much, but
>definitely some.
>
>Chuck, since I got ambivalent murky looking extracts after trying to
>separate the oil soluble from the alcohol extraction, I repeated the
>extractions using hot water first.
>
>There is quite a bit of greenish yellow pigment in the water extract.  I
>did several water rinses to make sure all of that pigment was out, then
>did a warm isopropyl alcohol extraction and got more of the same color.
>Then added paraffin oil, shook it up, and got some of the same color in
>both layers.
>
>??
>
>I think this is too conceptually messy for me, with me using different
>(less volatile/toxic/flammable) extractants that don't work the same way
>as the extractants you are using.
>
>The pigment distribution was the same in standards and falls with both
>extractions - the first time, I used falls without beards or hafts, &
>got more pigment from the standards.  The second time, I didn't remove
>the beards, and got about the same concentration of pigment from each.
>
>Hope these two extractions give you the answers you wanted - sure
>confused the heck out of me!
>
>
>- --
>Linda Mann east Tennessee USA zone 7/8
>East Tennessee Iris Society <http://www.korrnet.org/etis>
>American Iris Society web site <http://www.irises.org>
>talk archives: <http://www.hort.net/lists/iris-talk/>
>photos archives: <http://www.hort.net/lists/iris-photos/>
>online R&I <http://www.irisregister.com>
>
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