Re: Re: CULT: microscopy
- Subject: Re: [iris-talk] Re: CULT: microscopy
- From: o*@aol.com
- Date: Fri, 4 Oct 2002 13:41:28 EDT
In a message dated 10/4/2002 7:45:27 AM Central Daylight Time,
lmann@volfirst.net writes:
> Do you fix them in something or stain or ? Are iris leaf cuticles thick
> enough to see with a regular light microscope or does that take more
> magnification?
>
I have a Swift M1000D microscope. I purchased it at a time I was doing scape
conversion of diploid daylilies to tetraploid. At that time I used it to
determine the ratio of dip to tet pollen grains. Too, at that time I was
contemplating meristem tissue culture as a solution to rapid increase of
promising seedlings (too much work).
In those instances where I have examined iris rhizome sections I did not dye
the sections nor did I take any action to preserve samples - there being a
seemingly enless supply of both rhizomes and soft rot here. Have never
sectioned a leaf and examined. I have no idea what capacity of scope is or
what power is recuired to examine cell tissue. Best pics are obviously from
electron scopes. Can't afford one of those....yet.
As a side note, I found that I could use a slide projector, an overhead
projector, or a microfish card reader more efficiently than a microscope to
make the daylily pollen grain ratio counts. Of these devices the microfish
reader was the most convienent.
Science here is more of the kitchen table variety than of the applied
research genara here. I understood and appreciated your humor - slicin' and
dicin'. Thinkin' hybridizin' to be more like dicin' though some like poker
too. <g>
Bill Burleson
[Non-text portions of this message have been removed]
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