Re: SEED GIVEAWAY - CORRECTION
In a message dated 04/13/2000 8:41:40 AM Eastern Daylight Time,
greatpumpkin99@hotmail.com writes:
<< I did the
testing myself on a sample of the seeds (mixed and pulverized) from this
pumpkin using bioindicator innoculation, which is very accurate, even for
detecting unknown or rare viruses or other pathogens affecting cucurbits.
The tests came out clean, and ruled out any cucumovirus, potyvirus, etc.
This was the same test I used to detect the CMV present in foliar and sap
samples last season in my other plants.
If it would make anyone feel better (or maybe just for my personal
satisfaction of making certain people here eat their words), I might send
another sample to a professional testing facility to screen using a
double-antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA)
process, and a hybridization process where RNA and DNA are extracted (used
to detect viroids), as well as a test for phytoplasmas using a polymerase
chain reaction process, where an enzyme is used to copy DNA to detect if any
phytoplasmas are present. This would cost me A LOT, and is beyond overkill,
since the plant had no virus symptoms at all, and especially since CMV is
not even reported (not even once) to be transmitted via seed in c. maxima
whatsoever, but HEY, why not.... It would be fun having an official document
to flash around.
-Steve Sedlar
_____________________________________________ >>
What text book or encyclopedia did this get plagiarized from???
---------------------------------------------------------------------
Pumpkin-growing FAQ: http://www.mallorn.com/lists/pumpkins/search.cgi
To sign-off this list, send email to majordomo@mallorn.com with the
message text UNSUBSCRIBE PUMPKINS
