Nic Welty Seeds
- To: pumpkins@mallorn.com
- Subject: Nic Welty Seeds
- From: H* E* P*
- Date: Wed, 21 Apr 1999 19:03:41 -0700
Dear Nic Welty and others interested
Your seeds arrived today. Thanks for the superb information you sent
with them. Such monster vine diameters!
I entered the info on the URL below which will show the work as it
progresses this summer. Comments on my protocols are always valued.
I am particularly interested in eating quality and Nic selected seeds
for this goal.
One of the fruits was grown from a colchicine treated seed. I will
particularly observe plants from that fruit for any evidence of
colchicine effects. Colchicine sometimes increases chromosome numbers.
It has a reputation for doubling chromsome numbers, but I am not sure it
does that dependably.
I am aware one always reads about colchicine being used on seeds.
However, we need to keep in mind that we are primarily interested in
modifying the chromosomes of the germline cells, but where is that in a
huge AG seed? Is the embryo so advanced in development that the germline
is a large number of cells? Perhaps one needs to excise the apical
meristem and treat just that and the rescue it in tissue culture.
If attempting that, I would first get the pumpkin growing in culture
on sterile medium and then attempt the chromosome modifications.
I have never read a protocol for colchicine treatment or talked to
anyone who has done it. I have talked to others who knew only what they
had heard and they had not heard any more than me.
--
Harold Eddleman Ph.D. Microbiologist. i*@disknet.com
Location: Palmyra IN USA; 36 kilometers west of Louisville, Kentucky
http://www.disknet.com/indiana_biolab/pk711.htm <= Seeds from Nic Welty
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