Re: Cloning top lines.

Harold Eddleman replies to to messages:

> I am glad someone finally is taking this seriously! The only problem 
> with cuttings is that it wouldn't be a true full clone, since it would 
> just be a vine of the actualy plant, even though it can survive 
> independanlty. A full cloning of the embryo would be the ideal way 
>  to run some REAL experiments.
>
> Like I said, in one season we could learn more about the AG genetics 
> than we could in 10-20 years any other way. 
  HE: I disagree. The vine clones are the ideal clone. When working with
embryos one does get deformities. When sweetpotato is propagated by vine
cuttings all the progeny have perfect orange color. 
  When one uses storage roots to get slips via somatic embryogeneisis
(sp) some off color roots are produced. I agree this is not excatly the
embryo that you had in mind. I will not accept your embryo premise until
you provide research reference supporting your view.
  The use of vine and other cuttings has worked for thousands of years
and in nature for millions of years (willow trees, onion bulblets, etc). 
  I agree with you that clones greatly improve research and I support
you fully in you efforts to bring this suggestion to AG research. When
you looks a field of any crop produced by cuttings (The superb cloninc
method) from a single mother plant one sees that the plants do differ
due to local fertility, pruning, etc. as you have been saying. 

Now moving on to Glenn"s comment:

I doubt that you would ever get a cutting from a AG to root anyway.
I believe it is because of the high moisture content of the plant.

HE: I have never tried, but I will. It need not be a tip cutting. Folks
say AG roots at each node. Once I thinks he has a superior fruit, he
could let a side vine root and then cut it from the parent vine. Then
comes the problem of how to keep the vine alive during winter. There is
also the problem of whether the vine will get old and die. Recall apple
trees get old and die, but if you uses grafting he can maintain the
clone for hundreds of years. 

Tissue culture is anoher way to maintain clones of plants. As in my
business. We supply table sugar for carbon and energy. We add N-P-K-and
all the other needed minerals. A few vitamins and inositol are also
needed. Cool white fluorescent lights spaced one tube every 12 inches
and 16 or 20 inches above the shelf works well and I am able stack the
trays 3 deep. 
-- 
Harold Eddleman Ph.D. Microbiologist.       i*@disknet.com 
Location: Palmyra IN USA; 36 kilometers west of Louisville, Kentucky
http://www.disknet.com/indiana_biolab




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