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Hardening off

I started some seedlings about March 20 or so and they were started in 
flats with plastic domes over a hot pad (about 85 degrees).  Some came 
up within two days. (keep in mind that most of these are tropicals, 
(such as cassia, Argyrea nervosa, Daubentonia, Senna, wild hibiscus from 
Florida, etc) and there were some Asclepias in there, too.  Well, the 
seedlings came up & I repotted, sprayed with Wilt-Pruf, and put them in 
the greenhouse (with night temperatures of about 60 degrees F).  They 
didn't seem to be doing so well, so I brought them in the house & put 
them under lights.  I have lost some of the tropicals with very small 
leaves but the ones with thicker leaves seem to be taking the move much 
better.  Is there any hard & fast rule on the best way of hardening off 
seedlings you remove from under a clear plastic dome?  Thank you very 
much, Judy Showers (Greenhouse grower in Pennsylvania, zone 6)
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