SR HYB Mutagenesis two
- To: sibrob
- Subject: [sibrob] SR HYB Mutagenesis two
- From: W* C* D* <1*@compuserve.com>
- Date: Wed, 18 Oct 2000 22:57:42 -0400
Hello everyone!
After some internet research and discussion with a local high biology
teacher with a Masters in Botany ( she also worked on a UV irradiation
experiment as luck would have it!), I can say that UV irradiation is a low
cost and viable mutagenesis process a careful layperson could trial.
UV-A, UV-B and UV-C irradiation experiments have been conducted since the
late 1940's. Flower parts, pollen, seeds, seedlings, and tissue cultured
callus have been irradiated and mutations have routinely occurred with many
different types of plants; rye, corn, nicotiana, arabidopsis. (best results
were achieved by irradiating tc callus)
One experiment has shown that irradiating pollen can improve viability.
Different results have been achieved by irradiating with UV vs. xray or
gama rays due to the different nature of the irradiation and the fact that
plants have the ability to repair ( sometimes with mixed results) UV-A and
UV-B irradiation.
It is not a effective as x-ray or gamma irradiation, but you can't buy an
x-ray system for $10-$15.00 like you can a UV-C germicidal lamp.
With the information Dennis has shown me about Surflan/Treflan as a
polyploidy agent, I feel informed enough to try some experiments next
summer. Presently the seed I have are too precious with which to
experiment.
This could lead to different lines of hybridizing activity; one standard
methodology following the typical crossing methods of hybridizing. Another
line inducing diploids to tetraploidy and yet another using mutagenesis.
Bill Dougherty
Fridley, MN.
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