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Re: [aroid-l] Hybrids and doubling chromosomes


You might want to use treflan instead of colchicine.  As far as creating
tetraploids specifically, it would be much more efficient and safer.  Colchicine
has its uses too, but treflan would be your best bet.  Can forward a link
on procedures if you need it.  Don't know if its been tried on aroids or
not, but don't see why it wouldn't work.  

Tim C.

>-- Original Message --
>From: <plantguy@zoominternet.net>
>To: <aroid-l@lists.ncsu.edu>
>Subject: Re: [aroid-l] Hybrids and doubling chromosomes
>Date: Fri, 21 Nov 2003 19:50:32 -0500
>Reply-To: aroid-l@lists.ncsu.edu
>Hi Brian,
>Sounds like an interesting project.  We use colchicine all the time to
>disrupt microtubules and look at their role in vesicular trafficking of
>channels in my lab, but I never before looked into it in terms of plants.
>After reading your post and then a few articles I guess I'd say what the
>heck, it's so easy why not give it a whirl.  Obviously, all you'd need
>enough seed/plant material to try many concentrations and various time
>points.  I would caution that if you don't know what your doing with toxins
>like this it wouldn't be a wise choice.  Hopefully you have a good
>analytical balance and a hood for working with these types of compounds.
> If
>you're planning on using DMSO as a carrier I'd hope you would double
>glove....better safe than sorry.  Perhaps this would be a fun project for
>next spring in the lab.  It will be interesting to see what others familiar
>with plant cell biology have to say!
>Dan Devor
>----- Original Message -----
>From: "Brian Williams" <pugturd@alltel.net>
>To: <aroid-l@lists.ncsu.edu>
>Sent: Friday, November 21, 2003 6:23 PM
>Subject: [aroid-l] Hybrids and doubling chromosomes
>> I am interested in hearing if anyone has been doing hybrid work with
>> Aroids. Extremly interested in the storing of pollen in freezers or
>> other ways. Would be interested in exact temps time periods and results.
>>  I have also heard of Hybrid amorphophallus though never seen a pic.
>> Does this thing exist and if so why is their no info or pics of it. I
>> understand the botanist point of view but from a collectors its very
>> interesting.
>> Last but not least has anyone tried chromosome doubling? Using chemicals
>> on aroids like Colchincine? I have been reading up on it. It seems very
>> interesting. Has anyone ever tried or working with this or other types
>> of chemicals to produce tetraploids?

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