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Re: Sterilizing spore & agar as a growth medium.

  • Subject: Re: Sterilizing spore & agar as a growth medium.
  • From: bob needham <needham.rider@gmail.com>
  • Date: Mon, 25 Jan 2010 08:12:49 -0800
  • Dkim-signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=gmail.com; s=gamma; h=domainkey-signature:mime-version:received:in-reply-to:references :date:message-id:subject:from:to:content-type; bh=+ssfwIs/6t+sYo5XtX+da6gOeVv6ZqhBlF1hyBAt/+U=; b=KXCnCJt/NyccuayLqIlK+IFn7TZk1HvBeoqS/2bPYkzewW7r9MGg1PQtybW3DX1lEI 1+pcDj5KyoAZTdQcIiz9CWpMa/w8rIZpt1JzfaAJv2ifSsNRLDv/gaIVYbM3yWwh2kCl UVXJA2M+Gr2Oh1ngGWeu7/uy/m0hqnrXMbnAo=

Nice!
Makes sense.
-Thanks,
-BN

...nice to e-meet you.

On Mon, Jan 25, 2010 at 7:25 AM, MATT BUSBY <matt4u@btopenworld.com> wrote:

> For all but the most experimental reasons in a lab, there is no reason to
> sterilise spores. It is more important to clean spores so that they are
> free
> of dross (ie sporangial debris) This could introduce other spores of fungi
> etc.
>
> If you are simply growing ferns from spores for horticultural purposes, it
> is
> better to sow on a peat-based compost. It can be difficult to establish
> ferns
> in compost that have been germintated on agar.
>
> Matt Busby
>
> --- On Sun, 24/1/10, Darren Lloyd <Darren@ferntastic.com> wrote:
>
>
> From: Darren Lloyd <Darren@ferntastic.com>
> Subject: [ferns] Sterilizing spore & agar as a growth medium.
> To: ferns@hort.net
> Date: Sunday, 24 January, 2010, 22:47
>
>
> Hello,
>
> Do any of you sterilize your spore prior to sowing it, and if so could you
> tell me how please?
>
> I have read about various methods, including alcohol.
>
> Also, do any of you use agar as an initial growth medium?
>
> It seems to be used fairly widely, but is there any real advantage in doing
> so.
>
>
> Thanks!
>
> Regards,
>
> Darren Lloyd
>
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