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Re: Spore sieve(s) / spore refrigerator / digital pH meter.


The sieves came in on Tuesday. I finally ran the Pentagramma pallida spore through last night and certainly cleaned up what came through the 90 micron (170 mesh). I probably need to use a dissecting micro to verify how much besides the spore came through but it should work well enough. I'll run the P. triangularis var. maxonii through over the weekend and get spore off to you the beginning of the week.

Give me call some evening and we can discuss culture for the xerics, what little they need at least.

David Schwartz
Bakersfield, CA
USDA Zone 9(A)
Sunset Zone 8

-----Original Message-----
From: Darren Lloyd <Darren@ferntastic.com>
To: ferns@hort.net
Sent: Thu, 11 Sep 2008 12:37 pm
Subject: RE: [ferns] Spore sieve(s) / spore refrigerator / digital pH meter.


Thank you for your reply!

This is great information. How have the new sieves worked out for you?

As for pH, I'm not thinking of using pH testing for propagation purposes,
but rather for my planted/potted tree ferns mainly. I like to keep the pH in
the 5.5-6.5 range for these ferns.

Actually, if you don't mind it would be very useful to discuss cultural
requirements for xeriphytic ferns.



-----Original Message-----
From: owner-ferns@hort.net [mailto:owner-ferns@hort.net] On Behalf Of
Sent: Friday, August 29, 2008 7:50 AM
To: ferns@hort.net
Subject: Re: [ferns] Spore sieve(s) / spore refrigerator / digital pH meter.


I'd have to agree with Sue regarding the "need" for a sieve to sort spore. I
generally use the paper tapping method myself and end up with fairly clean
spore. That being said, I have used a series of graduated sieves to get rid
of large detritus, or if I know I have lots of trash that I want to go

Sometimes I'm collecting spore from older, dried fronds that have shed most
of their spores but, given the amount and type of indument on the frond,
will tend to always have some spore clinging to them. In this case, I often
crush the fronds in a?marble mortar and pestle, and then run them through
the sieves. I'll also do this with Pellaea mucronata, and P. brachyptera, as
the pinnules can close before shedding all or most of the spore when being
dried.?The small sieve set I have currently, has 30, 80 and 100 mesh
screens. The 100 mesh equates to 150 micron openings. 

Given what limited information I could find regarding spore size in
Cheilanthoid ferns (Flora of North America-Vol 2, The Pteridophytes of
Mexico), it seems that most of the spore is less than 100
microns?(Astrolepis cochisensis var. arizonica and A. cochisensis var.
chihuahuensis both--87 microns or smaller). This would equate to about a 140
mesh (106 micron). 

I will note that occasionally, I've ended up sowing whole sporangia (because
that's mostly what was left when don
e tapping) and had good germination from
them. If I had used a sieve with a 140 mesh in that case, I would have had
much less success in the germination...and no, I can't remember with what
particular fern species that occurred. 

The spore that I'll be sending you should be pretty clean. I try to be
diligent, especially when sharing spore I've collected. I'm not sure if
issue with the farina and Pentagramma pallida spore will be addressed unless
you can get down to the 100 micron level. The other option is to wait till
later this winter or spring and allow me to try to collect fresher spore,
and maybe that will come with less of the farina.

Regarding pH; I've found that for spore sowing I don't have to worry too
much about the pH, even with lime loving ferns like Argyrochosma jonsii and
A. limitanea. In fact, I had very poor luck when trying to incorporated
ground limestone into the sowing media for A. jonesii the first couple of
times I tried to sow it. The first time I used my standard mix, I had great
germination so I stopped worrying about it. Regardless, I've included a link
to 'Forestry Suppliers, Inc' http://www.forestry-suppliers.com/?that has a
wide range of pH testers (and a wide range of prices that match). They also
have some sieves. 

Good luck,

David Schwartz
Bakersfield, CA

-----Original Message-----
From: Darren Lloyd <Darren@ferntastic.com>
To: ferns@hort.net
Sent: Thu, 28 Aug 2008 7:43 pm
Subject: [ferns] Spore sieve(s) / spore refrigerator / digital pH meter.



I have a few fern related question please:


1) I'm planning to purchase a sieve/sieves for processing my spore. Does
anyone have any recommendations as to the mesh size(s) best suited for the


2) I'm also thinking of purchasing a small spore refrigerator. Does anyone
have any recommendations in this regard?


3) I'm also looking for an inexpensive digital pH meter with a soil probe,
but haven't managed to find one yet.


Thanks in advance!



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