Re: Hormones
- To: hosta-open@mallorn.com
- Subject: Re: Hormones
- From: J* H* <h*@gcnet.net>
- Date: Sat, 06 Feb 1999 22:26:31 -0500
- References: <abd64dc8.36bd015d@aol.com>
HoroRL@aol.com wrote:
>
> Jim:
>
> Thanks for the information about the vitamins and their uses in tissue
> culture. When you sterilize a tc media, to what log reduction would you go
> for it to be considered sterile? Also, what temperature do you use? Are you
> concerned about vitamin B and its analogs thermoliability?
>
>
-----------------------------------------------
>Rich,
Protocol for sterilization of media is at least 15 minutes at 15 #/sq
in. pressure in cooker. Under these conditions some ingredients may
become destroyed or reduced in potency. For amateurs like me, I must
accept whatever happens because I do not have vacuum filter
sterilization equiptment. If I had such, I would add any ingredients
that may become destroyed by heat AFTER autoclaving.
What is this, a test? Your questions are becoming more and more
difficult!!!
I will have to ask Beth Arnold to help me out.
Jim
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