Several years ago I had the pleasure of touring Pat
Scolnik's tissue culture lab, Breeze Hill Farm.
It's much more difficult to harden off small tissue
culture explants than it is seedlings.
Pat had a real interesting technique. She had
plastic domes that covered her flats that allowed her to go from full dome cover
to domes that had a few holes in them to domes that had more holes in them to
domes that had lots of holes in them and finally to no dome at
It was a real neat system and Pat was using it on
hundreds of flats of hosta tissue culture liners. Pat was using this system to
root stage four plantlets(just leaf....and no roots) and was rooting stage four
hosta into promix like soil Vs. the regular agar medium used in tc labs for
stage four rooting. She said her losses were a little higher but it eliminated a
whole stage in the hosta tc process.
If you use the regular size greenhouse flats the
plastic domes are cheap and it's easy to afford many domes for different
Dan & Lu