Re: Iris pumila, a different photo


Most impressive, Ken.

Rick Rodich - Minnesota

On Fri, 13 Mar 2009 10:16:15 -0700 Kenneth Walker <kenww@astound.net>
writes:
> I decided to try my hand at chromosome counting a couple years ago.
> After a long search I found a company that would sell aceto-carmine
>
> solution to an individual (the acetic acid component is 9 times
> stronger
> than vinegar, so the liquid and fumes are a bit caustic - most
> suppliers
> don't want the liability). Event then the process proved more
> difficult
> than I had hoped. After a number of attempts, yesterday I managed to
> get
> a picture of one cell where the chromosomes were separated enough
> and I
> managed to get the microscope focused just right so that I can count
> 30
> chromosomes. The photo is attached; the scale is about 16 pixels per
>
> micron. I had to look at huge number cells to get that one photo.
>
> I grew the Iris pumila from seeds, SIGNA 03A011. I. pumila comes in
> 30 &
> 32 chromosome varieties where the 32 variant has 4 sets of 8
> chromosomes. I assume the 30 chromosome version has lost a pair of
> chromosomes at some point.
>
> Ken Walker
> Concord, CA
>
>
>
>
> ------------------------------------
>
> Yahoo! Groups Links
>
>
>
>
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