Re: Bacillus thuringiensis


From: "Mark, Maureen" <MARKM@tc.gc.ca>

John,

I used to culture B. thuringiensis var israelensis many years ago.  I
don't remember all the details but I do recall blood agar (possibily
enriched with tryptophan) and warmer than room temperature.

Maureen Mark
/*@ottawa.com
m*@ottawa.com
Ottawa, Canada (zone 4)

> ----------
> From: 	John Montgomery[SMTP:monashee@junction.net]
> Reply To: 	iris-talk@onelist.com
> Sent: 	Sunday, July 19, 1998 5:16 PM
> To: 	iris-talk@onelist.com
> Subject: 	[iris-talk] Re: Digest Number 19
> 
> From: John Montgomery <monashee@junction.net>
> 
> 
> 
> Carryl Meyer wrote:
> 
> > reply to Doreen about thinking that spraying with BT kills the
> bees--
> > I spray about every two weeks--apple trees which are located above
> my
> > bee hive--I usually spray in the morning while the hive is in the
> > shade and the bees are not warm enough and not up and about
> yet--have
> > been doing this for many years and have not killed my bees yet!  (I
> > really do not like to use chemicals and since BT is a Bacterium I
> > rationalize that using it is better than using a chemical)---but
> even
> > if it were a chemical--use common sense--if you think it kills
> > bees--do not spray when bees are about!  When I do not spray often,
> I
> > get cherries and apples filled with worms--yuk!
> > Thanks for replies about rot--Carryl in western Montana
> > carrylm@bigsky.net
> 
> Yes, it is correct that BT is safe with bees. It works well against
> leaf
> roller which is fast becoming a major pest in apples here. I also use
> it
> for tent caterpillars or perhaps more correctly fall web worm.
> 
> I have two questions:
> 
> Carryl, what is the timing for spraying cherries with BT? I have
> almost
> made the decision to treat my two sweet cherries with the chainsaw
> remedy.
> 
> Secondly, I think I read at one time the instructions for preparing a
> culture in which to grow Bacillis thurengensis and thus keep a culture
> going. Does anyone have this information at hand?
> 
> Cheers
> 
> John Montgomery
> monashee@junction.net
> Vernon  BC  Zone 5
> 
> 
> 
> 
> 
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